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. Author manuscript; available in PMC: 2022 Sep 22.
Published in final edited form as: Sci Immunol. 2019 Nov 8;4(41):eaax7006. doi: 10.1126/sciimmunol.aax7006

Figure 8. G-CSF directly augments TH2 cytokine production from human ILC2s.

Figure 8

(A) Human ILC2s were isolated from peripheral blood and expanded in bulk culture with IL-7 (5 ng/ml) and IL-33 (15 ng/ml), before the addition of media or media containing 100 ng/ml recombinant G-CSF for 72 hours. (B) Relative expression of CSF3R was assessed by qPCR after 72 hours. (C) The levels of IL-5 and IL-13 in the ILC2 supernatant were assessed by a multiplex cytokine assay and expressed as a donor specific fold change after G-CSF treatment. (D) Expression of IL5, IL13, and GATA3 was assessed by qPCR after 72 hours, and presented as a donor specific fold change after G-CSF treatment. Results depicted as mean ± SEM. *P<0.05, **P<0.01, ***P<0.001 using a paired t-test.