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. Author manuscript; available in PMC: 2023 Mar 1.
Published in final edited form as: Nat Struct Mol Biol. 2022 Sep 1;29(9):881–890. doi: 10.1038/s41594-022-00820-9

Fig. 5. Phosphomimetic mutations result in exposure of target lysines in D2-I.

Fig. 5

(a-c) Monoubiquitination assays of 2 μM D2-IWT and D2-I3D complexes performed in the absence of DNA (a), in the presence of 0.1 μM 44-bp dsDNA (b), or in the presence of 5 μM 44-bp dsDNA (c). FANCD2 ubiquitination was analyzed using SDS-PAGE and Coomassie staining. (d) Quantification of FANCD2 monoubiquitination assays shown in panels a-c. Data obtained from three independent assays were quantified as percentage of ubiquitinated FANCD2. Replicate data were fitted using a global non-linear regression function described in the Methods. Data points of each replicate (symbols), the corresponding mean (central solid line) and the 95% confidence interval (shaded area between dashed lines) are shown.