Figure 1. Contribution of BcTol1 to virulence and ROS detoxification of B. cinerea.
(A) Relative level of BcTol1 transcript during the infection stage. The expression levels were normalized to that of the B. cinerea Actin gene.
(B) Amount of BcTol1 during the infection stage. Total protein was extracted from mung bean leaves inoculated with the BcTol1-GFP strain driven by the native promoter at the indicated times and probed with anti-GFP antibody. B. cinerea Actin gene was used as the loading control. The amount of BcTol1 at 6 hpi was set as 1.
(C) Acetylation of BcTol1 during the infection stage. BcTol1-GFP pulled down from the indicated samples was probed with anti-K122ac and anti-GFP antibodies. The amount of acetylated BcTol1 at 6 hpi was set as 1.
(D) Virulence of B05.10 and BcTol1 mutant strains on mung bean leaves. Photographs were taken 4 days after inoculation, and the diameter of disease lesions was measured for 30 infected leaves from 3 replicates of each strain.
(E) Sensitivity of B05.10 and BcTol1 mutant strains to H2O2. Photographs were taken 36 h after incubation on PDA medium with or without 20 mM H2O2, and the rate of inhibition of mycelial growth was measured for 3 plates of each strain.
(F) DAB staining shows ROS accumulation in mung bean leaves after infection by B05.10 and BcTol1 mutant strains. DAB staining was performed 24 h after inoculation, and the relative density was measured for 10 infected leaves of each strain.
See also Figures S1 and S2.