Fig. 3. The ribosome-bound intermediate states are partially folded.

a, The ¹⁹F NMR spectra of FLN5 + 37 RNC in the absence and presence of 2 M urea. Fractional populations shown below. b, The ¹⁹F NMR spectra of FLN5 + 47 and FLN5 + 47 Y719E RNC. Below, the line-width of FLN5 + 47 Y719E is compared against the line-widths of U determined for other RNCs (mean ± s.d.; Fig. 2f). c, The ¹⁹F NMR spectra of FLN5 + 47 and FLN5 + 47 P742A RNC. Analysis shown in Extended Data Fig. 4. d, The ¹⁹F NMR spectra of tfmF655-labelled FLN5∆6 + 47GS and FLN5 + 42GS RNCs (283 K, 500 MHz). Schematic depicts RNC construct design. Analysis shown in Extended Data Fig. 4. Unless stated otherwise, error bars indicate errors propagated from bootstrapping of residuals from NMR line-shape fittings.

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