Fig. 6. Free energy landscape and proposed model of co-translational folding of FLN5.
a, Free energy landscape of the co-translational folding of FLN5. Fractional populations determined by 19F NMR (Fig. 2e) were used to determine the difference in free energy between X (I1, I2 or N) and U (∆GX–U); in RNCs where U was not populated, an upper or lower bound of ∆GX–U was determined based on the spectral noise. The ∆GN–U of FLN5 + 110, and emergence from the tunnel (by solvent accessibility of the native, C-terminal C747 of FLN5; shaded region) were determined by PEGylation15,17. Error bars indicate errors propagated from bootstrapping of residuals from NMR line-shape fittings. b, Schematic of a proposed model for the co-translational folding mechanism of FLN5.