Table 2. Cytokine/chemokine detection in bronchoalveolar lavage fluid (BALF) after HDM challenge at ZT11 and ZT23 in WT and Rev-erbα-/- mice.
| BAL cytokines/chemokines increased in response to HDM | ZT11 (pg/ml) Mean (± SEM) | Adjusted P value |
ZT23 (pg/ml) Mean (± SEM) | Adjusted P value |
||
|---|---|---|---|---|---|---|
| WT | Rev-erbα-/- | WT | Rev-erbα-/- | |||
| ENA79/CXCL5 | 1708 (175.5) | 2193 (273.5) | 0.68 | 2097 (299) | 2012 (424.7) | 1.00 |
| IP-10/CXCL10 | 1418 (498.6) | 2017 (549.6) | 0.88 | 1793 (593.6) | 1798 (626.6) | 1.00 |
| SDF-1a/CXCL-12 | 59.33 (9.92) | 158.7 (69.29) | 0.53 | 72.58 (16.32) | 120.2 (40.5) | 0.98 |
| BCA-1/CXCL13 | 2158 (448) | 3088 (780.7) | 0.76 | 2025 (310.6) | 4670 (1179) | 0.03 |
| SCYB16/CXCL16 | 298.2 (51.95) | 157.9 (25.75) | 0.14 | 250.9 (37.11) | 204.8 (48.24) | 0.87 |
| RANTES/CCL5 | 49.91 (14.27) | 55.28 (27.11) | 0.055 | 36.95 (6.43) | 25.62 (7.14) | 0.56 |
| MCP3/CCL-7 | 12.65 (2.99) | 12.48 (3.98) | 1.0 | 20.25 (3.26) | 16.06 (4.82) | 0.86 |
| CCL-17 | 4430 (1681) | 5023 (1789) | 0.61 | 6006 (2560) | 3318 (1693) | 0.49 |
| MIP3b/CCL-19 | 94.13 (16.04) | 110.3 (26.16) | 0.96 | 99.79 (14.22) | 133.6 (30.55) | 0.76 |
| MIP-3a/CCL20 | 129.3 (32.64) | 102.9 (30.86) | 0.84 | 117.9 (17.49) | 165.2 (53.54) | 1.00 |
| MDC/CCL-22 | 347.8 (63.46) | 274.3 (81.15) | 0.93 | 498.1 (111.3) | 291.5 (77.17) | 0.31 |
| Eotaxin 2/CCL24 | 16520 (3078) | 25488 (8946) | 0.69 | 24546 (5616) | 15254 (9173) | 0.17 |
| IL-1b | 76.98 (14.17) | 82.18 (13.68) | 0.99 | 97.59 (9.86) | 89.1 (18.32) | 0.97 |
| IL-6 | 17.82 (3.41) | 12.48 (2.23) | 0.68 | 19.04 (3.17) | 17.74 (4.64) | 0.99 |
| IL-16 | 178.8 (21.3) | 175.2 (23.79) | 1.00 | 239.7 (36.24) | 279.8 (69.31) | 0.90 |
Cytokines/chemokines that increased significantly in BALF after intranasal HDM challenge are shown in the table. Cytokine/chemokine concentrations (pg/ml) for WT and Rev-erbα-/- mice after HDM challenge at ZT11 and ZT23 are shown as mean ± SEM. BCA-1/CXCL13 showed a genotype effect and was increased in Rev-erbα-/- mice at ZT23 compared to WT mice (* P < 0.03). P values are given in the table. 1 way ANOVA, followed by Tukey multiple comparison adjustment, n=8-12 per treatment group.