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. Author manuscript; available in PMC: 2022 Oct 5.
Published in final edited form as: Sci Signal. 2022 Jul 12;15(742):eabm5995. doi: 10.1126/scisignal.abm5995

Fig. 6. RNF12 is required for efficient germ cell differentiation in vitro.

Fig. 6

(A) Scheme for differentiating 2i mESCs to epiblast-like cells (EpiLCs) and primordial germ cell–like cells (PGCLCs). (B) Quantification of the indicated mRNAs in Rlim+/y 2i mESCs, EpiLCs, and PGCLCs by qRT-PCR. Data represented as mean ± S.E.M. (C) Quantification of the indicated mRNAs in Rlim+/y and Rlim-/y 2i mESCs differentiated to PGCLCs. Data was normalized to Rlim+/y mESCs and represented as mean ± S.E.M. Statistical significance was determined by paired T-test; confidence level 95%. (D) Quantification of the indicated mRNAs in Rlim+/y, Rlim-/y and Rlim-/y; Zfp42-/- mESCs differentiated to PGCLCs. Data was normalized to Rlim-/y; Zfp42-/- mESCs and represented as mean ± S.E.M. Statistical significance was determined by paired T-test; confidence level 95%. For all panels, n = 3 independent experiments.