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. Author manuscript; available in PMC: 2022 Oct 20.
Published in final edited form as: Nat Struct Mol Biol. 2022 Sep 12;29(9):891–897. doi: 10.1038/s41594-022-00821-8

Figure 2. Auxin mediated degradation of CTCF and SCC1.

Figure 2

(a) Flow cytometric analysis of GFP fluorescence (a.u.) in wild type, CTCF-AID6 and SCC1-AID25 cells after 6, 24 and 48 hours of auxin treatment. (b) GFP fluorescence of untreated (- auxin) and treated (+ auxin) CTCF-AID and SCC1-AID cells after 24 and 6 hours of growth in auxin, respectively (scale bars = 100 μm). (c) Hi-C heatmaps of the Shh TAD from untreated (- auxin) and 48 hour treated (+ auxin) CTCF-AID mESCs (16-kb resolution, datafrom ref 6). Genes, CTCF ChIP-seq tracks and CTCF sites 1-5 from ref 18 are shown above. CTCF ChIP-seq data from untreated (left) and auxin-treated (right) CTCF-AID cells are from ref 6. Insulation scores are shown below the Hi-C heatmaps (d) Hi-C heatmaps of the Shh TAD from 6 hour auxin treated TIR1 control or SCC1-AID mESCs at 20-kb resolution (data from ref 25).