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. Author manuscript; available in PMC: 2022 Oct 20.
Published in final edited form as: Nat Struct Mol Biol. 2022 Sep 12;29(9):891–897. doi: 10.1038/s41594-022-00821-8

Figure 4. Gene activation from a close enhancer is not affected by cohesin depletion.

Figure 4

(a) Percentage of (left axis) Shh and (right axis) Lmbr1 expressing alleles, assayed by RNA FISH, in TALE-transfected SCC1-AID cells either untreated (- auxin) or treated with 6 hours of auxin (+ auxin). Cells were transfected with tSBE6-VP64 or tSBE6-VP64 -Δ. Data shown are from one biological replicate. Data from an independent biological replicate are shown in Extended Data Fig. 4a. The data were compared using a two-sided Fisher’s Exact Test, n = numer of alleles scored, ns – not significant (p>0.05). Source data Fig4. (b) Images from representative nuclei from tSBE6-Vp64- and tSBE6-Δ-transfected SCC1-AID cells - & + auxin showing DNA FISH signals for Shh/SBE6 probes. Scale bars: 5 μm. (c) Violin plots showing the distribution of interprobe distances (μm) between Shh/SBE6 probes in tSBE6-Vp64- and tSBE6-Δ-transfected SCC1-AID cells - & + auxin. **p<0.01, ***p<0.001, ****p<0.0001 (two-sided Mann-Whitney U-tests). Values for number of alleles scored, mean and inter-quartile distances are in Extended Data Fig 4c.