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. Author manuscript; available in PMC: 2022 Oct 27.
Published in final edited form as: Nature. 2022 Sep 15:10.1038/s41586-022-05319-3. doi: 10.1038/s41586-022-05319-3

Extended Data Figure 2. Negative-stain immunoelectron microscopy and immunoblotting of sarkosyl-insoluble material.

Extended Data Figure 2

PER4 was used at 1:50 in (a-c). (a), PD (Cingulate cortex); (b), PDD1 (Cingulate cortex); (c), DLB3 (Cingulate cortex); Syn303, Syn1 and PER4 were used at 1:4,000 in (d-f). The brain regions used for cryo-EM were also used for immunoblotting. The arrow points to the position of monomeric α-synuclein.