Fig. 6. Red pulp macrophages retain LSCs in the spleen.

Spic^wt/wt donor LSK cells were transduced with BCR-ABL1-GFP and transplanted into Spic^wt/wt and Spic^–/– mice. The leukemic compartment of the CML mice was analyzed 15 days after CML induction. A Spleen weight. Absolute numbers of (B) total leukemic cells, (C) LSCs, (D) LT-LSCs, (E) ST-LSCs in spleen. One representative experiment out of 3 is shown with n = 5–6 mice per group. F Frequency of L-lin⁻ cells in the spleen and BM. G, H Frequency of spleen LT- and ST-LSCs. One representative experiment out of 3 is shown with n = 5–6 mice per group. I 10⁵ total spleen cells from CML mice were plated into methylcellulose and BCR-ABL1-GFP⁺ colonies were enumerated after 7 days. Fold change (FC) numbers of colonies from Spic^wt/wt and Spic^−/− spleen cells are shown. One representative experiment out of 2 is shown with n = 6 mice per group. J Incorporation of BrdU in LSCs of the BM and spleen of Spic^wt/wt and Spic^–/– CML mice. One experiment with n = 8 mice per group is shown. K Total leukemic cells in the BM of Spic^wt/wt and Spic^–/– CML mice. Pooled data from 2 independent experiments with n = 10–11 mice per group is shown. FC of cell numbers of L BM LSCs, M LT-LSCs and N ST-LSCs. O 10⁵ total BM cells from CML mice were plated into methylcellulose and BCR-ABL1-GFP⁺ colonies were enumerated after 7 days. FC of numbers of colonies from Spic^wt/wt and Spic^–/– BM cells are shown. One representative experiment out of 2 is shown with n = 5 mice per group. Data are displayed as mean ± SEM. Statistics: Unpaired student’s t test.