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. Author manuscript; available in PMC: 2023 Apr 24.
Published in final edited form as: Nat Neurosci. 2022 Oct 24;25(11):1505–1518. doi: 10.1038/s41593-022-01179-2

Extended Data Figure 7. BLA neuronal silencing reduces dominance and altruistic choices.

Extended Data Figure 7

a, Left, average rank change after CNO or vehicle injection in mice that received hM4D in the BLA (two-sided Wilcoxon matched-pairs signed-rank test, p=0.0002; vehicle n=10, CNO n=13). Right, rank changes in each hM4D-expressing mice after i.p. injection of CNO. b, Number of hM4D-expressing mice, grouped by social rank, that showed rank change following CNO injection. c, Left, control and mice that received hM4D for BLA silencing were injected with CNO (3mg/kg) 30 minutes before the SDM task. At least 1 hour after daily session, mice were tested in the tube test for assessment of social ranking within cage mates. Right, cage composition. Each cage hosted 2 actor-recipient pairs. Actor mice received hM4D or control virus in the BLA. All the recipients received the control virus. d, Number of dominant or subordinate actor mice compared to their recipient conspecific (n=19; two-sided Fisher’s exact test p=0.1789). e, Social dominance (normalized David’s Score) quantified based on the number and directionality of interactions in the tube test in actor grouped by control (n=9) and hM4D (n=9) mice (two-tailed paired t-test: t=2.15, d.f.=14, p=0.0493). f-g, Social dominance (normalized David’s Score) quantified based on the number and directionality of interactions in the tube test in (f) control (two-tailed paired t-test: t=1.30, d.f.=16, p=0.2120) and (g) hM4D actor mice (two-tailed paired t-test: t=1.331, d.f.=14, p=0.2045). h-i, Rank positions of (h) control and (i) hM4D mice in each cage over testing days. j, Number of altruistic and selfish choices in subordinate (grey, n=6) and dominant (red, n=4) mice that received hM4D in the BLA and CNO at the end of training and during the five days of SDM task (two-way ANOVA, group (dominant, subordinate), F(3, 80)=3.9, p=0.0107). k, Decision preference scores in the SDM task of hM4D-injected animal, grouped by dominant and subordinate. *p<0.05. Values are expressed as mean ± s.e.m.