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. Author manuscript; available in PMC: 2023 Apr 24.
Published in final edited form as: Nat Neurosci. 2022 Oct 24;25(11):1505–1518. doi: 10.1038/s41593-022-01179-2

Figure 5. BLA neuronal silencing abolishes the preference for altruistic choices.

Figure 5

a, Representative images and bar graph quantification of c-fos expression in mice following the last day of SDM task and number of c-fos-positive cells in mice tested with or without a recipient (n=42 sections from 7 animals, 3 independent experiments; two-tailed unpaired t-test: t=2.13, d.f.=40, p=0.0394). Scale bar (applicable to all micrographs), 50 μm. b, Male mice were bilaterally injected in the BLA with AAV-CamKIIa-mCherry (control, orange) or AAV-CamKIIa-hM4D-mCherry (hM4D, fuchsia). Representative image of a coronal section of BLA. c, 30 minutes before daily SDM session control and BLA hM4D mice received i.p. injection of CNO. As control, we also tested hM4D animals that received vehicle. Since we did not observe differences, we pooled the control animals together (two-tailed unpaired t-test: t=0.927, d.f.=8, p=0.3810). d, Left, decision preference score in the five days of SDM in control (n=9) and hM4D (n=10) mice (two-way RM ANOVA, group (control, hM4D) x time (days 1-5), F(12, 140)=1.981, p=0.0301; one sample t-test to chance (0.0), control: t=3.146, df=44, p=0.0030; hM4D: t=1.730, df=49, p=0.0899). Right, individual decision preference score in the SDM of control and hM4D. e, Average decision preference score across five day of SDM (two-tailed paired t-test: t=2.175, d.f.=17, p=0.0440) and number of control (n=9) and hM4D (n=10) mice displaying preference for altruistic or selfish choices. f, Number of altruistic and selfish choices in control (two-way ANOVA RM, choice (altruistic, selfish) x time (days 1-5), F(4, 64)=5.0, p=0.0013, n=9) and hM4D mice (choice (altruistic, selfish) x time (days 1-5), F(4, 80)=1.5, p=0.2024, n=10) over five days of SDM task. g, Representation of altruistic and selfish choices at the end of the training in the SDM task (day 5) in control (left) and hM4D (right) mice. *p<0.05, ***p<0.001. Values are expressed as mean ± s.e.m.