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. Author manuscript; available in PMC: 2023 Mar 5.
Published in final edited form as: Nat Chem. 2022 Sep 5;14(11):1295–1305. doi: 10.1038/s41557-022-01021-z

Extended Data Fig. 2. Evaluation of RNA substrate cleavage site preferences by the FR6_1 XNAzyme.

Extended Data Fig. 2

(a) Sequence of “FR6_1_NucSR”, a variant of XNAzyme FR6_1 retargeted to cleave RNA substrate “NucSR”(see reference (56)) by changing FANA residues shown in brown. (b) Urea-PAGE gels showing reactions of FR6_1_NucSR (5 uM) with all possible variants of RNA substrate NucSR (1 uM) at positions 8, 9 and 10 (shown in red in (a))(24 h, 17 °C, 10 mM Mg2+).