Fig. 4.

Mitochondrial function and glycolytic flux in luminal MCF‐7. (A) Respiratory rates expressed as flux control ratios in MCF‐7 cultivated in glutamine only media. (B) Combined complex II activities obtained from n = 6 biological replicates with MCF‐7 in full media in the Oxygraph‐2k with SEM. (C) Relative complex II activities in MCF‐7 cells grown in full media, glutamine only media, and glucose only media; n = 6 for full media, n = 4 for glutamine only and glucose only, respectively, with SEM. (D) Glycolytic activity based on proton efflux rates in MCF‐7 cultivated in full media, n = 6 biological replicates with SEM. (E) Relative extracellular acidification rates (ECAR) measured during the glycolytic stress test and (F) during the glycolytic rate assay in the Seahorse XF HS Mini; n = 6 biological replicates with SEM. Significance threshold for Students' t‐test: *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001.