Table 1.

List of chemicals and antibodies all chemicals were used according to standard operating procedures (SOP) and the safety data sheet provided by the company.

	Company	Cat. number
Cell culture
DMEM, 500 mL	Pan‐Biotech	P04‐01548
DPBS, 500 mL	Pan‐Biotech	P04‐36500
EMEM, 500 mL	Lonza	12‐125F
Trypsin, 100 mL	Sigma	T3924
FCS, 500 mL	Gibco	10270‐106
Dialysed FCS, 500 mL	Thermo	26400044
D‐Glucose, 100 mL, 2.5 m	Sigma	G8769
L‐glutamine, 100 mL, 200 mm	Sigma	G7513
Penicillin/Streptomycin, 100 mL	Sigma	P4333
MEM non‐essential amino acids, 100 mL	Pan‐Biotech	P08‐32100
Dimethylsulfoxide (DMSO)	Roth	4720.4
Sodium pyruvate, 100 mL,  100 mm	Sigma	S8636
Polybrene, 5 g	Sigma	H9268
Puromycin, 10 mg	Sigma	P8833
10 cm dish	Sarstedt	83.3902
6‐well plate	Sarstedt	83.3920
T175 flask	Sarstedt	83.3912
T75 flask	Greiner	658170
CASY® cell counter and  analyser	Schärfe System	Model DT
High‐resolution respirometry, O2K
Malonic acid, 5 g	Sigma	M1296
di‐Sodium succinate, 100 g	Sigma	8.18601
ADP sodium salt, 5 × 1 g	Sigma	A2754
Digitonin	Sigma	D141
Rotenone	Sigma	R8875
Antimycin A	Sigma	A8674
Seahorse XF HS Mini
XF Calibrant Solution, 100 mL	Agilent	103059‐000
XF DMEM Medium pH 7.4,  500 mL	Agilent	103575‐100
Seahorse XF HS Mini FluxPak	Agilent	103723‐100
Glycolytic Stress Test Kit	Agilent	103017‐100
Glycolytic Rate Assay Kit	Agilent	103346‐100
Wet lab
Skim milk powder, 500 g	Sigma	70166
Tween® 20, 100 mL	Sigma	P1379
Magnesiumchloride‐Hexahydrate, 1000 g	Merck	1.05833.1000
Potassium‐dihydrogen‐phosphate, 1000 g	Roth	3904.1
HEPES, 250 g	Sigma	H3375
Taurine, 100 g	Sigma	T0625
Lactobionic acid, 25 g	Sigma	153516
Sucrose, 1000 g	Sigma	S0389
Bovine serum albumin, 100 g	Sigma	A7030
EGTA, 10 g	Roth	3054.1
Bradford Protein Assay, 450 mL	Bio‐Rad	500‐0006
Immonilon® Western,  HRP Substrate	Millipore	WBKLS0500
Pierce BCA Protein Assay Kit	Thermo	23227
Annexin V/PI Apoptosis Detect.  Kit	BD‐Biosciences	556547

	Company	Cat. number	Host	Dilution
Primary antibodies
hFAHD1  (polyclonal)	FPLC purified from rabbit serum	–	Rabbit	1 : 1000
ß‐Actin	Sigma	A5441	Mouse	1 : 20 000
α‐tubuline	Sigma	T5168	Mouse	1 : 10 000
GLS (KGA/GAC), polyclonal	Proteintech	12 855‐1‐AP	Rabbit	1 : 1000
Complex I  (NDUFA9)	Invitrogen	459 100	Mouse	1 : 2000
Complex II (SDH)	Invitrogen	459 200	Mouse	1 : 20 000
mPEPCK	Santa Cruz	sc‐32 879	Rabbit	1 : 2000
GAPDH	Proteintech	10 494‐1‐AP	Rabbit	1 : 5000
Secondary antibodies
Polyclonal  swine‐anti‐rabbit	DAKO	P0399	Swine	1 : 3000
Polyclonal  goat‐anti‐mouse	DAKO	P0447	Goat	1 : 20 000