Skip to main content
. Author manuscript; available in PMC: 2023 May 10.
Published in final edited form as: Nat Methods. 2022 Nov 10;19(12):1558–1562. doi: 10.1038/s41592-022-01658-1

Extended Data Fig. 5. MCP-GFP-PAB1* can be used to image MBS-tagged ATP2 mRNAs.

Extended Data Fig. 5

(A) Live-cell imaging of yeast cells expressing MCP-GFP-PAB1 (left) or MCP-GFP-PAB1* (right). Images were max-Z projected. Scale bars are 2 μm. The cell outline is marked with a white dashed line. (B, C) Sequence alignment of PAB1 homologs flanking the two conserved phenylalanines, which are F170 and F366 in yeast PAB1 (F142 and F337 in human PABPC1). These two phenylalanines were mutated to valines in PAB1*/PABPC1*.