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. Author manuscript; available in PMC: 2022 Dec 10.
Published in final edited form as: Biomaterials. 2022 Jan 4;281:121363. doi: 10.1016/j.biomaterials.2022.121363

Fig. 5. Effect of BMP receptor and β chain integrin silencing on cell adhesion and spreading.

Fig. 5

C2C12 cells were transfected with siRNA against BMP receptor type I (ALK2, ALK3, ALK5, ALK6), BMP receptor type II (BMPR-II, ACTR-IIA, ACTR-IIB) and β chain integrins (β1, β3, β5) and plated on soft (light color) and rigid (dark color) films with bBMPs for 5 or 4 h, respectively. The cell number par mm2 of substrate area and the spreading area were quantified. The relative % is given, in comparison to a control scrambled siRNA. (a) Relative cell number (%), (b) relative cell area (%). Data represent the mean ± SEM, with 3 biological replicates and 2 technical replicates per experiment. Statistical tests were done using non-parametric Kruskal-Wallis ANOVA test (*, p ≤ 0.05; **, p ≤ 0.01). Statistical comparisons were made between soft and rigid films for each condition.