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. Author manuscript; available in PMC: 2022 Dec 14.
Published in final edited form as: Nat Cardiovasc Res. 2022 Nov 11;1:1056–1071. doi: 10.1038/s44161-022-00155-0

Figure 6. Impact of WT and Gpr55-/- adoptive B cell transfer into global Apoe-/- mice on atherosclerotic plaque formation and Ig titers.

Figure 6

(A) Circulating and (B) splenic leukocyte counts in Apoe-/-Gpr55-/- mice treated with the B cell depletion cocktail or control animals treated with the respective isotype antibodies (n=3 per group; ***p<0.0001). (C) Average body weight (n=6), (D) total plasma cholesterol (n=6), (E) IgG (n=6; p=0.0125) (F) and IgM titers after 4 weeks Western diet (WD). (G) Aortic root plaque size (n=6; p=0.021), and (H) representative oil red O-stained sections of B cell-depleted female Apoe-/- mice receiving WT or Gpr55-/- B cell adoptive transfer and subsequent WD feeding for 4 weeks; here we show one representative picture of the six per group that were evaluated. (I) Splenic B cell subsets quantified by flow cytometry (n=6). Data were obtained in one experiment (mean ± SEM; n=6 per group). Two-sided unpaired Student’s t-test was used to determine the significant differences..