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. Author manuscript; available in PMC: 2023 Jun 8.
Published in final edited form as: Nature. 2022 Nov 16;612(7939):347–353. doi: 10.1038/s41586-022-05426-1

Extended Data Fig. 2. (related to Figure 2): Dying tumor cells activate mTORC1 in surrounding tumor cells in vivo.

Extended Data Fig. 2

a, Immunofluorescence analysis of p-S6 (red) and EGFP (Lgr5, green) expression in tumor tissues of vehicle or DT treated AOM/DSS mice 24h after injection. Nuclei were counterstained with DAPI. Representative images are shown (n=3 biological replicates, scale bar = 50 μm).

b, Immunoblot analysis of AOM/DSSATKN tumor organoids treated as indicated. For p-γH2AX and the corresponding α-tubulin loading samples were run on a separate gel (see SI). Representative results are shown (n=3).