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. Author manuscript; available in PMC: 2022 Dec 16.
Published in final edited form as: Nat Cardiovasc Res. 2022 Oct 12;1:918–932.

Fig. 2. Oestrogen signalling is mediated via oestrogen receptor 1 (Esr1) and G-protein coupled oestrogen receptor 1 (Gper1).

Fig. 2

Representative confocal images of Type-H (Emcnhigh CD31high) vessel changes upon endothelial-specific deletion of oestrogen receptors (Esr1, Esr2, Gper1) at P28 in mice. Graphs indicate quantifications of total ECs (CD31+ CD45-Ter119-) by flow cytometry for Esr1 (Control n=11F, n=7M; Mutant n=9F, n=6M), Esr2 (Control n=4F, n=4M; Mutant n=6F, n=5M), Gper1 (Control n=6F, n=8M; Mutant n=6F, n=7M); quantification of Type-H vessel volume per field of mp for Esr1 (Control n=11F, n=7M; Mutant n=9F, n=6M), Esr2 (Control n=4F, n=4M; Mutant n=6F, n=5M), Gper1 (Control n=6F, n=8M; Mutant n=7F, n=10M); and quantification of Type-H EC area normalised to mp area for Esr1 (Control n=11F, n=7M; Mutant n=9F, n=6M), Esr2 (Control n=4F, n=4M; Mutant n=6F, n=5M), Gper1 (Control n=6F, n=6M; Mutant n=6F, n=9M). Data are mean ± s.d. for image quantification and mean ± s.e.m. for flow cytometry data; Two-Way ANOVA with Tukey’s test. Scale bars 40μm.