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. Author manuscript; available in PMC: 2022 Dec 18.
Published in final edited form as: Exp Cell Res. 2022 Feb 25;414(1):113084. doi: 10.1016/j.yexcr.2022.113084

Table 2. Overview of utilized antibodies for Western-blot.

Antibody Isotyp catalogue number kDa manufacturer dilution
anti-N-cadherin IgG1 61 09 20 130 BD/Biosciences 1:1000
anti-E-cadherin IgG2a 610,181 120 BD/Biosciences 1:2500
anti-β-catenin IgG1 61 01 53 92 BD/Biosciences 1:4000
anti-HSP-70 IgG2b 818,101 70 Biolegend, California, USA 1:200
anti-Slug Rabbit IgG 9585 30 Cell Signaling, Danvers, MA, USA 1:1000
anti-KLF4 Rabbit IgG ab215036 54 Abcam, Cambridge, UK 1:1000
anti-GAPDH IgG1 ab8245 36 Abcam, Cambridge, UK 1:5000
Fluorochrome secondary antibody
αmouse IgG2a 800 CW IgG2a 926-32351 LiCor Bioscience, Germany 1:5000
α mouse IgG1 680LT IgG1 926-68050 LiCor Bioscience, Germany 1:10,000
α rabbit IgG 800CW IgG 926-32213 LiCor Bioscience, Germany 1:5000
α mouse IgG IR800 IgG AC2135 Azure 1:2500
Peroxidase secondary antibody
α mouse IgG HRP IgG 31,430 Pierce, ThermoFisher Scientific 1:10,000
α rabbit IgG HRP IgG 31,460 Pierce, ThermoFisher Scientific 1:10,000