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. Author manuscript; available in PMC: 2022 Dec 30.
Published in final edited form as: ACS Appl Mater Interfaces. 2022 Mar 11;14(11):13107–13121. doi: 10.1021/acsami.2c01747

Figure 5. Cell adhesion, spreading and proliferation of PAN092 on soft (EDC30) and stiff (EDC70) PEM film.

Figure 5

Fluorescence imaging of cells with nucleus labelled with DAPI (blue) and actin cytoskeleton labelled with rhodamine (red) on (A) soft film and (B) stiff film with FN, BMP2 or BMP4. Cell adhesion (C, F, I), cell spreading (D, G, J), and proliferation (E, H, K) were examined in the presence of fibronectin (C-E), BMP2 (F-H), or BMP4 (I-K). The mean of 3 independent experiments is plotted with SEM. * indicates that the value is significantly different from the control condition.