Extended Data Fig. 3. Transcriptional changes upon dTAG47 treatment.

(a) Heatmap of ATAC and ChIP-seq signal measured as log₂ fold-change for genomic regions in the five WT SMARCA4^dTAG time-course clusters. (b) Volcano plot of nascent transcriptional changes after 3h dTAG47 treatment in WT SMARCA4^dTAG cells (PRO-seq). Significant changes (Padj < 0.01 and abs(log2 fold-change) >1) are colored in red. Two-sided Wald test was performed, False discovery rate (FDR) correction as implemented in DESeq2. (c) PCA plot of variance stabilizing transformation normalized counts from the RNA-seq experiment in WT SMARCA4^dTAG cells. (d) Heatmap of ATAC and RNA-seq signal measured as log₂ fold-change for genomic regions in the five WT SMARCA4^dTAG time-course clusters. (e) Volcano plots of gene expression changes after dTAG treatment in SMARCA4^dTAG cells compared to DMSO treatment and in SMARCA4^KO cells compared to WT cells as measured by RNA-seq. Significant changes (Padj < 0.01 and abs(log2 fold-change) >1) are colored in red. Two-sided Wald test was performed, False discovery rate (FDR) correction as implemented in DESeq2.