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. Author manuscript; available in PMC: 2023 Feb 1.
Published in final edited form as: Sci Immunol. 2022 May 13;7(71):eabl6543. doi: 10.1126/sciimmunol.abl6543

Figure 5. IL-13 activates BMP signaling in stem cells, skewing them away from tuft cell progenitors.

Figure 5

A, Time points for RNAseq experiment from small intestinal organoids treated with 10 ng/mL cytokine at indicated timepoints and with and without 5 μM DMH1 for 24 hours. B, Volcano plots comparing indicated treatments from experiment described in A. C, D, Gene expression of experiment described in A. Each circle is one biological replicate, statistics calculated with DESeq2, see methods. E, scRNAseq data of small intestinal organoids treated with IL-13 and untreated from Biton et al (3). F, RNA velocity analysis of tuft cell populations from scRNAseq data presented in E. G, H, Violin plots of gene expression in tuft cells from scRNAseq data presented in E. I, See C. J, Gene expression of small intestinal organoids treated with indicated treatment for 72 hours. Each circle is one biological replicate, statistics calculated with DESeq2, see methods. TPM = transcripts per million, CPM = Counts per million.