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. Author manuscript; available in PMC: 2023 Feb 10.
Published in final edited form as: Methods Mol Biol. 2022 Jan 1;2438:217–230. doi: 10.1007/978-1-0716-2035-9_14

Figure 1. Schematic to demonstrate positioning of embryos for single cell and tissue laser ablation.

Figure 1

A) For single cell ablations, the caudal region of the embryo is cut using forceps and positioned by piercing the ventral half of the tissue using a curved suture needle. The tissue is then held in place with both ends of the suture needle fixed in the agarose. B) For tissue level ablation, whole embryos are positioned by creating a hole in the agarose and piercing the embryo through the body to the walls of the hole using a suture needle.