Fig. 8. Cells were cultured in six-well plates supplemented with 5% (wt/vol.) BSA or 750 μmol/l palmitate with or without rosiglitazone (10 μmol/l) for 24 h followed by 2 h in the corresponding serum-free medium.

2-Deoxyglucose (2-DOG) uptake over 5 min was measured following stimulation with or without 100 nmol/l insulin for 15 min. Podocytes treated with palmitate had significantly reduced insulin-stimulated glucose uptake. Co-treatment with rosiglitazone and palmitate significantly increased both basal and insulin-stimulated glucose uptake by comparison with palmitate alone (*p < 0.05), but uptake did not fully recover to the insulin-stimulated uptake at 24 h. Data are expressed as percentage of vehicle basal uptake and the SEM is shown of five independent experiments, each performed in triplicate