Skip to main content
. Author manuscript; available in PMC: 2023 Mar 4.
Published in final edited form as: Kidney Int. 2022 Jun 15;102(4):815–827. doi: 10.1016/j.kint.2022.05.019

Figure 4. Type IV collagens are expressed in the zebrafish glomerulus.

Figure 4

A) Schematic (created with Biorender) showing the three type IV collagen alpha chains expressed in the glomerular basement membrane (α3, α4, and α5) on the left, and their interaction to form the α4α4α5(IV) heterotrimer on the right. B) In situ hybridisations showing the expression patterns of all six type IV collagen chains in zebrafish at 4 dpf. Panels on the left are lateral views of the whole embryo (anterior left, scale bar = 100 μm). Panels on the right are dorsal views of the head and anterior trunk region (anterior to the left, scale bar = 50 μm). Inlet panels in the col4a1 and col4a3 whole embryo profiles show the glomerulus in transverse section (scale bar = 10 μm) through the point in the embryo highlighted in a red dashed line on the whole embryo image. C) Panels show transverse sections through the glomerulus of 4 dpf zebrafish embryos immunostained for podocin (left panel) and pan-collagen IV (middle panel). Right panel shows schematic representation of the tissues in cross-section at this position.