Skip to main content
. Author manuscript; available in PMC: 2023 Mar 6.
Published in final edited form as: Leukemia. 2022 May 26;36(8):1969–1979. doi: 10.1038/s41375-022-01601-5

Figure 5. Deletion of Cxcl12 from mesenchymal stromal cells leads to cell cycle arrest and cell death in LSCs.

Figure 5

(A) Gene set enrichment analysis (GSEA) of the transcriptional signature of LSCs showing a depletion in signatures related to integrin signaling (left panel) and regulation of cell adhesion (right panel) in LSCs from the Prx1-Cxcl12-Mut mice (NES: Normalized enrichment score). (B) Frequency of LSCs in G0/G1 and S, G2/M phases of the cell cycle of LSCs from the Prx1-Cxcl12-Mut and Prx1-Cxcl12-Ctrl mice. (C) Cell death measured by Annexin-V, TO-PRO-3 staining in LSCs from the Prx1-Cxcl12-Mut and Prx1-Cxcl12-Ctrl mice. Data are representative of at least 2 independent experiments. n=6 mice per genotype per experiment unless otherwise stated. Data are represented as mean and SD. * P< .05; ** P< .01; *** P< .001.