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. Author manuscript; available in PMC: 2023 Mar 6.
Published in final edited form as: Leukemia. 2022 May 26;36(8):1969–1979. doi: 10.1038/s41375-022-01601-5

Figure 6. CXCL12 produced by mesenchymal stromal cells protects LSCs from oxidative stress-induced DNA damage and cell death.

Figure 6

(A) Mitochondrial membrane potential of LSCs isolated from the Prx1-Cxcl12-Mut and Prx1-Cxcl12-Ctrl mice. (B,C) Quantification of (B) ROS levels and (C) mitochondrial superoxide anion production of LSCs isolated from the Prx1-Cxcl12-Mut and Prx1-Cxcl12-Ctrl mice. (D) Representative images showing p-γH2AX (ser139) in LSCs isolated from the Prx1-Cxcl12-Mut and Prx1-Cxcl12-Ctrl mice and their quantification(right panel). (E) Cell death measured by Annexin-V, TO-PRO-3 staining in LSCs isolated from the Prx1-Cxcl12-Mut and Prx1-Cxcl12-Ctrl mice. (F) Kaplan-Meier curve showing survival of C57BL6J mice (n=12/group) transplanted with equal numbers of LSCs from Prx1-Cxcl12-Ctrl (n=23) and Prx1-Cxcl12-Mut (n=23) animals that were fed NAD or vehicle in the drinking water throughout leukemia development. NAC denotes the addition of N-acetyl-cysteine to the drinking water (1mg/mL), starting 2 days after leukemia infusion. Data are representative of at least 2 independent experiments; n=12 mice per genotype per experiment unless otherwise stated. Data are represented as mean and SD. * P< .05; ** P< .01; *** P< .001.