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. Author manuscript; available in PMC: 2023 Mar 20.
Published in final edited form as: Sci Signal. 2021 Jun 22;14(688):eabe6156. doi: 10.1126/scisignal.abe6156

Fig. 1. Several delayed down-regulated genes are lowly expressed in mammary tumors, relative to peritumor regions.

Fig. 1

(A) Heatmap of top 25 genes that were consistently down-regulated in MCF10A cells after 240- to 480-min-long exposure to EGF. Data are from GSE24391. (B) The ratio of gene expression in tumors versus expression in the respective peritumors of the four subtypes of breast cancer was analyzed using TCGA data. The heatmap presents the DDGs lowly expressed in at least one subtype of breast cancer. (C) Pathway overrepresentation analysis using MSigDB, Panther 2016, and KEGG databases. The group of 43 genes from (B) was used. Shown are the most significant pathways (P < 0.05). Note that “KRAS signaling Dn” refers to genes down-regulated by KRAS activation. (D) Real-time qPCR using mRNA isolated from EGF-stimulated MCF10 cells to assess transcript levels of three DDGs. Shown are fold changes relative to basal levels (means ± SEM of three independent experiments). GAPDH transcripts were used for normalization.