Fig. 3.

(A) Differentiated podocytes were cultured in the control medium or supplemented with 5% BSA (vehicle), 375 μM or 750 μM palmitate for 24 h. Ceramide production was determined by lipid extraction and thin layer chromatography. There was a significant increase in ceramide production in podocytes treated with 750 μM palmitate compared to control (one-way ANOVA, overall P = 0.0137, with post hoc Bonferroni, P < 0.05*). These data represent n = 2 repeats with each experiment performed in triplicate. (B) Differentiated podocytes were treated for 24 h with 5% BSA (vehicle), 750 μM palmitate or a combination of 750 μM palmitate with either fumonisin B1 or myriocin. 2-DOG uptake over 5 min was measured following stimulation with or without 100 nM insulin for 15 min. Both fumonisin B1 and myriocin significantly increased insulin-stimulated glucose uptake in palmitate-treated cells (one-way ANOVA, overall P < 0.0001 with post hoc Bonferroni, P < 0.001 with palmitate and fumonisin Bl* and P < 0.01 with palmitate and myriocin**). Data are expressed as % of vehicle basal uptake and the SEM is shown of n = 3 independent experiments each performed in triplicate.