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. Author manuscript; available in PMC: 2023 Sep 28.
Published in final edited form as: J Exp Bot. 2023 Jan 18;74(6):1940–1956. doi: 10.1093/jxb/erad020

Figure 7. MYB12 binds to TMO5 in yeast and tobacco leaves.

Figure 7

(A) Yeast-two-hybrid assay with pDEST22 (prey) or pDEST32 (bait) constructs containing fusion proteins of the MYB12 and TMO5 coupled to respectively, the activator (AD) or binding domain (BD). The yeast colonies are representative colonies of 24 independent yeast transformants per prey and bait pair. The empty pDEST22 or empty pDEST32 plasmids were used to check for auto-activation. Transformed yeast grown on the selective -Trp/-Leu (-T -L) medium and interaction verifying - Trp/-Leu/-His (-T -L -H) medium. (B) co-immunoprecipitation of TMO5-HA and LHW-LIKE1-HA with MYB12-GFP (full blot is shown in Figure S13). (C-D) Knock-sideways with Mito-FRB, TMO5-EGFP-FKGP and MYB12-TagBFP2 (C) or free TagBFP2 (D) as control in absence or presence of rapamycin. Arrows indicate the aggregated mitochondria and arrowheads indicate the nucleus. (n ≥10 for C and n ≥20 for D) Scale bars are 20 μm in C and 10 μm in D.