FIGURE 5.

Schematic representation of the most common styles of Organ‐on‐a‐Chip (OOC) technologies for the vasculature. The figure illustrates the general classification of channels: (A) straight single channels and (B) branching channels that often divide into channels of reduced size for a hierarchical structure. Then, the OOC styles are identified according to the cross‐section. (C) In hollow rounded channels, endothelial cells and smooth muscle cells can grow layered in the tubular area that resembles the vessel shape. (D) In hollow rectangular channels, endothelial cells usually attach to the bottom and/or top of the channel. In both hollow channels, it is possible to add circulating components in the flow, such as cancer cells or other cellular interactors. (E) Alternatively, single or multicellular components can be mixed with a hydrogel and loaded into a channel to establish a self‐assembled vasculature within days of cell culture. (F) and (G) Lastly, OOC models of the vasculature can be designed as multicompartmental devices with or without the membrane to regulate interactions between different cell types and environments. Source: Created with BioRender.com.