(A) Simplified representation of the results of the Markov chain analysis. Dashed circles, corresponding basal occupancies. See Table S3 for full transition probabilities.
(B) Changes in single-molecule state occupancies induced by isoproterenol (10 μM) stimulation. Data are normalized to the corresponding basal levels.
(C) Example of a βArr2 molecule undergoing a transient interaction with a β2AR to then reach a CCP without an accompanying receptor.
(D) Examples of βArr2 molecules visiting multiple CCPs via lateral diffusion.
See also Figures S3 and S4 and Table S5.