. Author manuscript; available in PMC: 2023 Jun 1.

Published in final edited form as: Nature. 2023 May 10;618(7963):188–192. doi: 10.1038/s41586-023-06050-3

Extended Data Table 1. Cryo-ET data collection and processing.

Parameters of cryo-ET data set collection used for subtomogram averaging, resulting in the STA map of bridge structures.

	ERMES bridges
	EMD-16873
Cryo-ET data collection and processing
Microscope	Titan Krios
Magnification	33000 ×
Voltage (kV)	300
Detector	Gatan K3 in superresolution mode
Energy filter	yes
Electron exposure per tilt series	approx. 140-150
(e^-/Å²)
Defocus range (μm)	-3.5 to -6.0
Pixel size (Å)	1.342
Tilt range (min, max, increment)	-56°, +56°, 1°
Tilt scheme	Dose-symmetric
Frame number	4
Tomograms used for STA (no.)	51
Initial subtomograms (no.)	1133
Final subtomograms (no.)	1098
Map resolution (Å) at FSC threshold 0.143	29
Map resolution range (Å)	-