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. Author manuscript; available in PMC: 2023 Jul 14.
Published in final edited form as: Nat Genet. 2023 Jun 8;55(6):973–983. doi: 10.1038/s41588-023-01408-9

Extended Data Figure 8. rs117643180 exhibits allelic differences in transcription factor binding.

Extended Data Figure 8

An EMSA using 6μg per lane of nuclear extract from undifferentiated LHCN-M2 cells shows protein-DNA interactions for probes centered around each both alleles of rs117643180. The probe containing rs117643180-A shows allele-specific protein binding (Arrow A, lane 6), relative to the probe containing rs117643180-C (lane 2). A 25-fold excess unlabeled probe containing the A allele competed away A-specific bands more effectively (lane 7) than 25-fold excess unlabeled probe containing the C allele (lane 8). Arrow B shows a biotinylated free probe (200 fmol per lane). Uncropped image is available in Source Data.