Extended Data Fig. 4. No difference in bone marrow leukocyte production between Tie2^WT and Tie2^iδAEC mice.

a. Peripheral blood cell counts indices in the Tie2^WT and Tie2^iΔAEC mice four weeks after Western diet. b-c. Quantification of BM cellularity and spleen weight/bodyweight in the Tie2^WT and Tie2^iΔAEC mice (n = 3 independent mice per group). d-e. Quantification of BM stem and progenitor cell numbers in the Tie2^WT and Tie2^iΔAEC mice (n = 3 independent mice per group). f. Quantification of CD3e+ T cells, B220 + B cells and CD11b + F4/80+ macrophages in the BM from the Tie2^WT and Tie2^iΔAEC mice (n = 3 independent mice per group). g. UMAP plots of (15) immune cell clusters from red blood cell-depleted total bone marrow of the Tie2^WT and Tie2^iΔAEC mice four weeks after Western diet with Immgen annotation. h. Quantification of immune cell clusters from the experiment. Values show mean ± s.d. Statistical significance was determined using Student’s t-test (two tailed, unpaired). i. Gene enrichment analysis showing pathways that are related the upregulated genes in the BM neutrophils and monocytes in the Tie2^iΔAEC mice. P value adjustment is performed using a Benjamini-Hochberg (BH)-procedure (Multiple comparison test) as indicated in the fgsea package. j. Quantification of CD11b + cells and CD11b + Gr1+ neutrophils in the BM from the Tie2^WT and Tie2^iΔAEC mice (n = 3 per group). Values show mean ± s.d. Statistical significance was determined using Student’s t-test (two tailed, unpaired).

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