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. 2023 Mar 13;2(3):307–321. doi: 10.1038/s44161-023-00224-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 2 — a. Representative images of β-gal staining in the thoracic aorta, b. aortic arch and c. aortic root from WT (Bmx^+/+) mice and in mice expressing the LacZ gene targeted into the Bmx locus. Scale bar, 1 mm (A-B) and 50 μm (C). d. Fold differences of Tie1 and Tie2 transcripts in the Tie1^iΔAEC (n = 3 independent mice per group, Tie1: P = 0.0025), Tie2^iΔAEC (n = 4 independent mice per group, Tie2: P = 0.0003), and Tie1;Tie2^iΔAEC mice (n = 5 independent Tie1;Tie2^WT mice and n = 4 independent Tie1;Tie2^iΔAEC mice, Tie1: P = 0.0003,Tie2: P = 0.0015) in comparison with their littermate controls. Statistical significance was determined using Student’s t-test (two tailed, unpaired). e. Immunofluorescence of Tie2 and PECAM1 in the aortic root, aortic arch and thoracic aorta of the Tie2^iΔAEC and Tie2^WT mice (n = 3 independent mice per group). Scale bar, 50 μm. f. Pcsk9-D377Y concentration in serum samples and body weight measurements at the indicated time points in the Tie2^iΔAEC and Tie2^WT mice (n = 6 independent mice per group). g. Quantification of cholesterol (CHOL) and triglyceride (TG) concentrations in serum samples from the Tie2-deleted mice (n = 6 independent mice per group) 20 weeks after Pcsk9 and Western diet. Values show mean ± s.d. Statistical significance was determined using Student’s t-test (two tailed, unpaired).

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