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. Author manuscript; available in PMC: 2023 Jul 21.
Published in final edited form as: Cell Microbiol. 2019 Nov 17;22(2):e13140. doi: 10.1111/cmi.13140

Figure 3. Transcriptional profiling of ifu5Δ/Δ cells.

Figure 3

(a) Flow chart indicating differential expression of genes in ifu5⍰/⍰. Boxes indicate list of functional categories according to GO annotation. Number of differentially expressed genes is indicated in brackets. (b) Quantitative polymerase chain reaction-based transcript analysis of Efg1 and its coregulated genes in ifu5Δ/Δ. Fold change (mutant/wild type) is calculated by 2-ΔΔCT, normalised to ACT1 (endogenous control) with untreated strain as calibrator. Values are means ± SD and are derived from three independent RNA preparations. A two-tailed, unpaired t test was used to determine the statistical relevance: ***p < .001