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. Author manuscript; available in PMC: 2023 Jul 31.
Published in final edited form as: Microbiology (Reading). 2022 Oct 1;168(10):10.1099/mic.0.001259. doi: 10.1099/mic.0.001259

Figure 3. Chromosomal parS16 arrays redistribute ParB to disrupt chromosome origin segregation.

Figure 3

(A) Origin localization in a wild-type strain and in a strain harbouring parS16 arrays. The oriC region was labelled using an array of tetO operators bound by TetR-GFP. Phase contrast (top panel), TetR-GFP (middle panel) and membrane dye FM 5-95 combined with TetR-GFP (bottom panel). (B) ParB dependent decrease in the number of origin per cell in the presence of parS16 arrays. Scale bar, 3μm.