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. Author manuscript; available in PMC: 2023 Aug 1.
Published in final edited form as: IEEE Sens Lett. 2023 Aug;7(8):1–4. doi: 10.1109/LSENS.2017.0000000

Fig. 1.

Fig. 1

(a) TMPRSS2-ERG RT-pHLAMP reactions conducted with 1 ng per reaction of mRNA extracted from prostate cancer cell lines. b The specificity of the TMPRSS2-ERG RT-pHLAMP reaction with synthetic fragments of TMPRSS2-ERG, TMPRSS2 and ERG mRNA tested at concentrations of 3 x 106 and 3 x 102 copies per reaction. (c) The standard curve for the AR RT-pHLAMP assay from 5 x 107 to 5 x 101 synthetic copies per reaction. (d) The pH change readout of the AR assay across serial dilutions varying from 5x107 copies to 5x101 copies per reaction. The starting pH of the AR RT-pHLAMP benchtop reaction was between 8.0-8.6 pH units for all recorded reactions.