Table 1. Canonical pre-mRNA 3′-end-processing factors in humans and budding yeast, their functions, and the multi-subunit protein complexes to which they belong. Alternative names are in brackets.

Human complex		Human protein	Function	Yeast protein	Yeast complex
CPSF	mPSF	CPSF160 (CPSF1)	Scaffold	Cft1	Poly(A) polymerase module	Core CPF	CPF
		WDR33	Scaffold, RNA binding	Pfs2
		CPSF30 (CPSF4)	RNA binding	Yth1
		hFip1	PAP/Pap1 recruitment	Fip1
	N/A	PAP	Poly(A) polymerase	Pap1
		RBBP6	Endonuclease activation	Mpe1	Nuclease module
	mCF	CPSF100 (CPSF2)	Pseudonuclease	Cft2
		CPSF73 (CPSF3)	Endonuclease	Ysh1
		Symplekin	Scaffold	Pta1	Phosphatase module	APT
N/A	N/A	SSU72	Protein phosphatase	Ssu72
	Phosphatase complex	WDR82	Transcription termination	Swd2
		PP1	Protein phosphatase	Glc7
		PNUTS	Scaffold, PP1/Glc7 activator	Ref2
		N/A	Scaffold	Pti1
		Tox4	DNA binding	N/A	N/A		N/A
	N/A	N/A	Ysh1 antagonizing	Syc1
	CStF	CStF50 (CSTF1)	Complex stabilizing	N/A		N/A
		CStF64 (CSTF2)	RNA binding	Rna15	CF IA
		CStF77 (CSTF3)	CPSF160/Cft1 binding	Rna14
	CFIIm	Pcf11	Pol II CTD binding	Pcf11
		Clp1	Polynucleotide kinase	Clp1
		N/A	Cleavage fidelity	Hrp1	CF IB
	CFIm	CFIm25 (CPSF5)	RNA binding	N/A	N/A
		CFIm68 (CPSF6)	hFip1 binding	N/A
		CFIm59 (CPSF7)	hFip1 binding	N/A
	N/A	PABPN1	Poly(A) tail binding	N/A
		N/A	Poly(A) tail binding	Pab1
		ZC3H14	Poly(A) tail binding	Nab2

Abbreviations: APT, associated with Pta1; CF, cleavage factor; CPF, cleavage and polyadenylation factor; CPSF, cleavage and polyadenylation specificity factor; CStF, cleavage stimulatory factor; CTD, C-terminal domain; mCF, mammalian cleavage factor; mPSF, mammalian polyadenylation specificity factor; N/A, not applicable; Pol II, RNA polymerase II; poly(A), polyadenosine; pre-mRNA, precursor messenger RNA.