Extended Data Figure 4. Immune cell homeostasis is not altered in Pou2f3^−/− tuft-cell-deficient mice.

a, The repartition of immune cells in wild-type and Pou2f3^−/− mice was monitored by flow cytometry. The presence of T (CD3⁺), B (CD19⁺), CD4⁺, CD8⁺, naive (Tn; CD3⁺ CD62L⁺CD44^-), central memory (Tcm; CD3⁺CD62L⁺CD44⁺), effector memory (Tem; CD3⁺CD62L^-CD44⁺), regulatory (Treg; CD4⁺Foxp3⁺), natural killer (NK; CD3^-NK1.1⁺) and myeloid (CD11b⁺Gr1⁺) cells was assessed by staining with fluorochrome-tagged antibodies and representative dot plots are shown. The percentages of positively-stained cells are indicated. b, Quantification of the different immune cells in lymph nodes (LN), mesenteric lymph nodes (mLN) and spleens (SP) of wild-type and Pou2f3^−/− mice are presented. Data are means ± s.d. (n = 3 mice per genotype). c, Total cells in LN, mLN and SP of wild-type and Pou2f3^−/− mice are presented as means ± s.d. (n = 3 mice per genotype). d, Immune lineage cells in the lamina propria of wild-type and Pou2f3^−/− mice were monitored by flow cytometry after tissue dissociation. The percentage of T cells was assessed within the CD45⁺ haematopoietic gate, CD4, CD8 and gamma-delta T cells (CD8⁺TCR-γδ⁺) within the CD3⁺ gate and myeloid cells within the CD3^- gate, as indicated. Representative dot plots are presented (left). Quantification of immune cells within the lamina propria are shown as means ± s.d. (n = 3 mice per group). No significant differences were detected for all cell types between wild-type and Pou2f3^−/− mice (P > 0.05). A two-tailed Student’s t-test was used.