Figure 2. Effects of PZQ on human schistosome specific antibody responses (adopted from ref ¹⁶).

2-dimensional Western blot analyses of serological reactivity of the treated cohort comparing pre- and post-treatment responses. The intensity of the IgG1 reactivity against adult schistosome worm antigens in serum pooled from 112 people (male and female) aged 5-42 was compare before and 12 weeks after treatment with the standard dose of Praziquantel (PZQ) of 40mg/kg body weight. (Full details are given in ref¹⁶)

a) Spots (antigens) reacting with sera collected at time 0 (before PZQ treatment). Boxes represent the position where additional spots absent in this Figure are present in Figure 2b.

b) Spots reacting with sera collected 12 weeks post-treatment. In addition to enhanced recognition of antigens recognised before treatment, additional antigens highlighted in the numbered boxes were identified.

c) Protein identities of the antigens identified by sera after treatment. The proteins highlighted in bold are on the current World Health Organisation list of schistosome vaccine candidates (see http://www.who.int/immunization/diseases/en/)