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. Author manuscript; available in PMC: 2023 Aug 10.
Published in final edited form as: J Vis Exp. 2020 May 28;(159):10.3791/61132. doi: 10.3791/61132

Table 3. List of RT-qPCR primers used in this study.

Gene Sequence Forward Sequence Reverse PCR efficiency (%) R2
ama-1 GGTGGATACAGCCCATCGAG CGAGGATGGAGTGTACGTCG 101 0.992
atg-1 CTGCCAAGACGGACTACGCT TGCTGCCACATACGACGAAG 148 0.849
atg-12 GGACACTGTTGCTTCCTTCATCT AAGAAGAGTGAGTTGTTGGCTTGA 97 0.923
atg-5 AAGACGAGTCGGCACAGTTGT CGATCGTCCAGAACTCGTCA 100 0.9599
atg-7 CAGCCACGACGATAATACAACAG CCACAAGCAACACATTGATCG 112 0.8555
atg-9 GGCTCATCCACAAGGTCACTG TCGGCAGGTGAATCGTGATAC 115 0.901
C12C8.1 TCGATGAAGTTGTCTTGGTTGG AGGCTACTGCTTCGTCTGGATT 110.1 0.978
C30C11.4 GAGACTCGTTCCAAGACTGATGC CGGCTTCTTCTTATTGAGGATAGG 109.51 0.997
cct-1 ACATCTCATTCACTTCCGACTCG CAACAACCAGCTCTCCGAAGA 107.88 0.99
cct-4 CAGCCACGACGATAATACAACAG CAGGCGGTAGAGCAGGAAGT 114.14 0.993
cct-5 CAGGAGAACCAGAAGCGTATCAC GACCGAGAGATGTGCGAAGAG 105 0.9944
cct-6 ACCACATCCACCGTTCTCCTT ACTCGAAGCCTTCAGTAACAATACG 102 0.9893
cdc-42 TCCACAGACCGACGTGTTTC AGGCACCCATTTTTCTCGGA 100.3 0.995
daf-16 GAACAGTGTCCGTGGATCGT CGGTAGTGGCATTGGCTTGA 100 0.9934
daf-21 ATTCGCTACCAGGCACTCAC TGGTAAGGGTCTTTTCCTCCT 109 0.994
dap-1 TGCCAGCTAAGATGGAAAGA ACACCGCTATTAGCATCACG 112 0.9942
dnj-11 AGCAAGCCGACAAGGAGACA CATCTCTCCACGGTTCCAGGT 107 0.9732
dnj-12 TCACTGCGACAGTTGTAATGGAG CCAGGCGTGATTCCAACTTC 105.81 0.99
dnj-13 CGGATAAGAATAAGGAAGCTGGAG AGTCCTTCCTCTCCGAATTGATC 103 0.98
dnj-15 TCGGTTGGATCAGGATGAGC TCTCCTCGTCAGTCGCCATT 112.15 0.98
dnj-16 AATATGATGAAGCTGTGGCGAA TTATACGGTTCTTGAGACTTCTGGAG 98 0.9062
dnj-19 TTCACGTTCTTCCTGGCATG GACAACATCTCCTGGCTCACC 98 0.9763
dnj-22 AAGAAGCCGAACGTAGATTCATTG GGTGTTCCTGAGCCATTCGA 101 0.9871
dnj-23 TAAGGCTGAGAAGGAGGCAATC AGTGAATCGAGGAACGACGTG 102.56 0.99
dnj-24 AATGGCGATGAACATATTGTTGA ATGTTGGCGGTGTTGTTACTGT 107.08 0.99
dnj-25 TTGACGAAGAACCTCCAGCAC TCCATTAGACCGAACGCAGAC 102.3 0.99
dnj-26 AGTACGCAAGGCGACGACAT AGGCTCATTGCTGGTAGAGTGTG 105.67 0.99
dnj-29 CGTTGCTTCAGCTTCCACAC ATGGCACGAGAATACACGCTT 100.09 0.99
dnj-30 TTGCTGATCGAGAGAAGAAGAGAA TTCTCTGCGGTCTGGTTGCT 106.71 0.99
dnj-4 CAGCGAACACATTACGAAGTGC CGGATGAGTTGTCAGGATGGA 95.37 0.99
dnj-5A CTCCATCTCAAGCAAGAAGTTGTAAG CGTGTAATAGGTAGAAGTCAATCCGA 102 0.9898
dnj-5B CTCCATCTCAAGCAAGAAGTTGTAAG CGTGTAATAGGTAGAAGTCAATCCGA 102 0.9834
dnj-7 GCACGGATAGTACGGACACCA CTTGAGGCATTCTCGGATAACG 110.61 0.99
dnj-8 TTCTCACAATATGTGTATCCTCTCAGAA CAGTTGGAAGTGTATGCTCCAGTAG 100.53 0.99
dnj-9 CGCAATTCTGAATGTTCCGA CAGCGTCCTTCTTCTCGTCAT 94.4 0.99
ERCC_171 CCATTGGATGAAGCCCAGGA CGTAATCAACGCCGCAACTT 109 0.995
ERCC_25 GGTTCGAGTACCCAGAGCAG AGCCGTAGACGCAACTCATT 91 0.932
ERCC_35 GGCTCTCTGATGCTACGACC CTACCGGTTACGACGCAGTT 78 0.9834
ERCC_42 CATGGTGAGGAGCTGGTGAG ATTGGAGGGCACTTACCTGG 103.7 0.998
ERCC_53 AACAAATCCTTTCCCTGCCTG TTGCTCACCAGCTACTGGAAAT 100.5 0.993
ERCC_60 TGTGTTCTTGTGTGAAGTGGC AAAAGAGGGGGAGGAGCCAA 82 0.9911
ERCC_9 GCTAGTCTCGCGCAGTACAT AGAGCTGCGTCACATACGTC 98 0.9894
F11F1.1 GCTGTTGGTGAGGATGGAGA TCATCATATCGCCGTCCAAT 100.8 9.979
F18C12.2 TCTTGCCAGAAGCGAGAACTC GATTGCTCATTGGTGGTGGAT 111 0.985
F21F3.3 CCATTTCTCCGAATTCGTCT GGGTAGAAGTTGGCCTCAAT 93.1 0.96
F44E5.4 GAATGGAAAGGTTGAGATCCTCG GCTGCATCTCCAACCAATCTT 97.8 0.992
F48D6.4b AAAGTGTCGAACCCTGTTCC GCATTGTCGGTCTTGTTCTTC 94 0.99
F55H12.4 TTGGCAAAGGAACCAGCTAT GTTTGCGAACCATACCACCT 105 0.964
gapdh TGGAGCCGACTATGTCGTTGAG GCAGATGGAGCAGAGATGATGAC 100.2 0.996
hsf-1 TGGTCTAACTCGAACAGAATCAGA TCCACAGTTCTTGCCGATTG 105.59 0.99
hsp-1 CACTGTTTTCGATGCCAAACG TCCTTCGGCAGAGATGACCT 107.76 0.988
hsp-12.3 TGGAGTTGTTAAGGTTCTGGACT CCAATGTTCTTCACATCAATCTCGT 95.54 0.999
hsp-16.1 ATGGCTCAGATGGAACGTCA TGGCTTGAACTGCGAGACAT 102 0.984
hsp-16.2 TCCATCTGAGTCTTCTGAGATTGTT TGATAGCGTACGACCATCCAAA 102.2 0.998
hsp-16.41 TGGGGAGATTGTAAATGATGAATCC TCAATTTTGAGCTCTCTTCCATCT 104.7 0.998
hsp-16.48/49 TGGATGAAATCACTGGATCTGT GATTCGTCATTTACAATCTCTCCAA 90 0.9952
hsp-3 CATCGCCTACGGACTTGACA AATGGTGAGCATGGATACATCG 106.63 0.98
hsp-4 TTCAACAAGACATCAAGCACTGG GGCAGAGACTTCTTCAGGAGTGA 96 0.9873
hsp-6 GATTGGATAAGGACGCTGGAGA CCGTTGGTGGACTTGACCTC 101 0.9916
ife-1 CAGCGTCTGGACTAAGGATTGC GGATCACATCGAACAGTGGCTT 108.43 0.99
ire-1 TACTTGCCACCACGGAGACC CGTTGCCATCGTCATCATTG 110.29 0.99
K07F5.16 AAGTGAAGGAGCACAACACGAAT GGAATGAAGCCACGAATCGT 111.6 0.99
let-363 AACTGATAAGACCAAGGAACGAGT GCGGAAGAACAAGATGAAGATG 110 0.962
lgg-2 CCGAGCATATAACCGTTGCC GCTTGTTGTGGATGAAGTTGGA 116 0.963
nlp-29 AGGATATGGAAGAGGATATGGAGG CTCCGTACATTCCACGTCCA 114.78 0.99
pha-4 GCACACTAGGAACCACGCAAG CCGAACTGTAGAGGTAAGGAGACG 100 0.963
phat-3 ATTGTGCTGTAAGACGGCTGACT CACTGTTCATTGGTAATGGTGGAG 95 0.8264
pmp-3 GTTCCCGTGTTCATCACTCAT ACACCGTCGAGAAGCTGTAGA 109.34 0.99
sip-1 CGGGTTCAGCAAGAGATCGT CCAAGTCGACGTCCTTTGGA 114.198 0.994
sma-10 AAGTTGCAAGTCTACCAAGCG CCGGTCTTGGAGTTCCTGTG 81.75
sma-3 TGGAAGCTCAACGGCATCTT TCGTGGTGAACTTGCACTT 106.6 0.987
stc-1 AATCAGTACCGAGTGTCGTTGCT TGTTCTTGTTGTTCCGTGGCT 96 0.9656
T02C5.1a AACGAGCGTGAAATCAAGTG GACTTTGCGATGAGCTGGT 111 0.971
T03F6.2 TTGTAGTGATGAATGTGATGAAGAAGG GATGTGTTGCTTCGAGTTCTCATG 112 0.99
T10B5.5 ATCGGAATCAAGAAGGTCAACG GAAGCCAGCATAACTGAACGC 101 0.9829
T14G8.3 TGTGCTGAATAAGGAGTCAAGAAGA GTAGGAAGATTGAGGATGCGAAG 101.39 0.99
T21B10.7 CTGGACTTGACTCGGCTGAAC CGTAACATCAGCAACCTCTCCTT 101 0.9772
T24H7.2 AGGTTGATGATGCTCCGAAGA AGCAGTTAGTGCTTCAACTGTATAGCTT 110 0.984
T28F4.5/ dap-1 TGCCAGCTAAGATGGAAAGA ACACCGCTATTAGCATCACG 108.45 0.998
ttr-45 CGACGGGCAAGGAATGTTCA CGGAGTCCTGGCTTCAACTT 114.15 0.99
vps-34 TTCTCACTTATGTTAGACGCTGGAAT TTGGTAGCTGCTTCATCCGA 92 0.907
Y22D7AL.5 CCAAGGACGTCAAGTTCGGA TCACGTTTCTTCCTTTTGGGC 106 0.9929
Y45F10D.4 GTCGCTTCAAATCAGTTCAGC GTTCTTGTCAAGTGATCCGACA 103.31 0.997
Y51F10.7 ATGCGAATTTGCACACAACT GTACGGCTTATTAGCAGCAATC 103 0.9825
Y54E10BL.4 TTCGCTGGAGCAAGACAACA TCTTGTGAAGCAGAATGGTCGA 106.02 0.96
Y55F3AR.3 GACACTGTGATTCTGTGGCTGTTC CGGATTATGATTGTGGCGACTT 95 0.984
ZK637.12.2 GATGGACAACGAGAAGCTGA GGGATTTGCATCTTCAACTG 92 0.927