Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
| Component | Volume (fraction) | Reactions - Equivalent to one 10 uL reaction | ||||||
|---|---|---|---|---|---|---|---|---|
| 1 | 3 | 5 | 10 | 50 | 100 | |||
| Extract (24 mg/mL) | 1/3 | 3.67 | 11 | 18.3 | 36.67 | 183.33 | 366.67 | Volume (uL) |
| DNA (80 nm) | 1/4 | 2.75 | 8.25 | 13.75 | 27.5 | 137.5 | 275 | |
| MES/SMM (2.4X) | 5/12 | 4.58 | 13.75 | 22.96 | 45.83 | 229.17 | 458.33 | |
| Total volume (uL) | 11 | 33 | 55 | 111 | 555 | 1110 | ||
Note: 10% extra (dead volume) is added to allow for pipetting error
