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. Author manuscript; available in PMC: 2024 Feb 1.
Published in final edited form as: J Control Release. 2023 Jan 13;354:305–315. doi: 10.1016/j.jconrel.2023.01.014

Figure 4.

Figure 4

Metabolic activity and plasma membrane integrity is not altered in activated and non-activated PCLS (three donors)as well as in primary CD4+ T cells after treatment with Tf-PEI and Tf-Mel-PEI polyplexes. (A) Evaluation of cellular viability by WST-1 assay in PCLS after transfection with 50 pmol siRNA. (B) Assessment of lactate dehydrogenase release by LDH assay following incubation with polyplexes in PCLS after transfection with 50 pmol siRNA and (C) primary CD4+ T cells after transfection with 10 pmol or 20 pmol siRNA. Data points indicate means ± SD.