Skip to main content
. Author manuscript; available in PMC: 2023 Aug 31.
Published in final edited form as: Cell Rep. 2021 Apr 6;35(1):108936. doi: 10.1016/j.celrep.2021.108936

Figure 3. Tom70/71 supports biogenesis of aggregation-prone mitochondrial proteins.

Figure 3

(A) The aggregation propensities (Conchillo-Sole et al., 2007) and the presence of iMTS-L sequences in proteins (Boos et al., 2018) were calculated. Plotted are the distributions of these values for Tom70-dependent (log2 FC, <—0.2) and -independent (log2 FC, >0.2) proteins.

(B) Aggregation propensities were calculated for different groups of mitochondrial proteins. The dotted line shows the mean value of Tom70-independent proteins as a reference.

(C) The indicated strains were precultured in galactose-containing medium at 30°C and spotted on galactose medium, following 3 days of incubation at 30°C, 34°C, or 37°C. WT, wild-type; ev, empty vector.

(D and E) The influence of temperature (log2 FC of Tom70 37°C as compared to Tom70 30°C) and the absence of Tom70 (log2 FC of Δtom70/71 as compared to Tom70 at 30°C) were analyzed. Blue circles show the isobaric distribution of mitochondrial proteins, whereas black ones show the distribution of the entire proteome. Enrichment of mitochondrial proteins among proteins with a log2 FC below a certain threshold was calculated, and significance of this enrichment was plotted (side panels).