Extended Data Figure 6. Notum drives the elimination of wild-type cells from the crypt independent of apoptosis.

a, Quantification and representative confocal imaging of cleaved caspase-3⁺ cells (CC3) within clonal crypts and the surrounding non-mutant epithelium in Notum^WT and Notum^cKO mice at 14 d.p.i. n=4 per genotype. Crypts adjacent (Adj.) to or remote from (Rem.) Apc-mutant (clone) crypts were scored as non-mutant epithelia. Red arrows indicate CC3⁺ cells (green) in areas of Apc-mutant clones (purple border) and surrounding epithelia. Scale bar, 50 μm. Data are mean ± s.e.m; Mann–Whitney two-tailed U-test.